Neuroprotective effects of Cubebin and Hinokinin lignan fractions of <i>Piper cubeba</i> fruit in Alzheimer’s disease <i>in vitro</i> model

Abstract Objectives The current research examines the protective effects of Piper cubeba ethanolic extract and its isolated lignans; Cubebin Hinokinin fractions against Alzheimer’s Disease (AD) in vitro model. Methods Dried powdered fruit P. were extracted ethanol fractionated using silica gel column chromatography. Of 15 eluted fractions, two indicated presence targeted Lignans; Cubebin. They monitored by thin layered chromatography their structures confirmed LC-HRMS spectrometry NMR analysis. Antioxidant activity crude lignan analyzed FRAP, DPPH ABTS assays. Anti-acetylcholinesterase was investigated β-amyloid (Aβ) cytotoxicity on SHSY-5Y human neuroblastoma cell lines studied MTT assay. Results showed similar if not significantly stronger antioxidant capacity compared to ascorbic acid FRAP Both lignans exerted weaker yet potent activity. yielded strongest acetylcholinesterase inhibitory potential however, there no significant difference (p&lt;0.05) between IC 50 values fractions. Significant neuroprotective μM Aβ at p&lt;0.05 observed for selected treated control. highly both 5 10 μg/mL. Hinokinin-containing improved viability SH-SY5Y cells only concentration 100 Conclusions from our studies suggest that these phytoconstituents might be good candidates prevention treatment AD.